THE POTENTIAL OF INTERACTION OF HYDROALCOHOLIC EXTRACT OF SEMECARPUS ANACARDIUM NUT EXTRACT WITH PROPRANOLOL IN ISCHEMIA-REPERFUSION INDUCED MYOCARDIAL DAMAGE USING ISOLATED RAT HEART

Abstract

The present study was carried out to determine the effect of hydroalcoholic extract of Semecarpus anacardium nut (SANE) and the possible interaction with propranolol (PRO) on experimentally induced myocardial infarction (MI) in rats. Isolated rat hearts were infused with propranolol 10 $\mu$ g/ml (PRO), SANE 1 $\mu$ g/ml (SANE 1), SANE 10 $\mu$ g/ml (SANE 10), SANE 25 $\mu$ g/ml (SANE 25), SANE 50 $\mu$ g/ml

(SANE 50) and SANE 10 + PRO dissolve in KH solution using modified Langendorff’s apparatus through the aorta and subjected to ischemic reperfusion injury (IRI) by stopping the KH flow. The influence of different treatment was analysed by quantification of % recovery in terms of heart rate and force of contraction, biomarkers and antioxidants in post ischemic condition and histopathological studies. The superoxide dismutase and catalase activities were reduced in the heart tissue of isolated heart perfused with PRO, SANE 1, SANE 10, SANE 25, SANE 10 +PRO compared with IRI control. The biomarker LDH activity decreases in perfusate with PRO, SANE 1, SANE 10, SANE 25, SANE 10+PRO treatment and rises in heart tissue homogenate (HTH) of SANE 10 and SANE 10+PRO group compared with IRI control. CKMB activity decreases significantly in perfusate of SANE 10 and SANE 10+PRO groups and elevated in HTH in the groups PRO, SANE 10, SANE 25, SANE 10+PRO as compared with IRI control. Significant level of % recovery in terms of heart rate and developed tension were observed in groups infused with SANE 10 and SANE 10+PRO compared with IRI control. Histopathological studies reported SANE 10+PRO as a best protective group. To conclude, perfusion of SANE 10 with PRO in combination demonstrated most significant cardioprotective efficacy in isolated rat heart subjected to ischemia reperfusion injury.