HIGH PERFORMANCE THIN LAYER CHROMATOGRAPHIC DETERMINATION OF DICLOFENAC SODIUM AND THIOCOLCHICOSIDE IN FIXED DOSE COMBINATION

Abstract

A simple, specific, accurate, and precise high-performance thin-layer chromatographic method for analysis of Diclofenac Sodium and Thiocolchicoside in fixed dose combination has been developed and validated. The method use aluminium plates precoated with silica gel 60 F₂₅₄ as stationary phase and Toluene: Ethyl acetate: Methanol (5:3:2, v/v/v) as mobile phase. Densitometric evaluation of the separated bands was performed at 285 nm. The two drugs were satisfactorily resolved with Rf values 0.53

± 0.005 and 0.17 ± 0.002 for Diclofenac Sodium and Thiocolchicoside, respectively. Results were found to be linear over the concentration range 50–300 ng/ band for both the drugs. Intra-day variation, as RSD (%), was 0.468 for Diclofenac Sodium and 0.357 for Thiocolchicoside. Interday variation, as RSD (%) was 0.949 for Diclofenac Sodium and 0.739 for Thiocolchicoside. This method has been successfully validated and applied for the analysis of drugs in pharmaceutical formulation. The % assay (Mean ± S.D.) was found to be 100.521 ± 0.833 for Diclofenac Sodium and 101.132 ± 0.612 for Thiocolchicoside.