PHARMACODYNAMIC INTERACTION OF GREEN TEA EXTRACT WITH HYDROCHLOROTHIAZIDE AGAINST ISOPROTERENOL INDUCED MYOCARDIAL DAMAGE IN RATS

Abstract

To evaluate the pharmacodynamic interaction of green tea extract with hydrochlorothiazide against isoproterenol induced myocardial infarction wistar albino rat of either sex were prophylactically treated with hydrochlorothiazide (10 mg/kg for 7 days), low and high dose of green tea extract (100 and 500 mg/kg for 3 weeks) and their combination by oral route. To induce the toxicity apart from normal control all other group subjected to isoproterenol (150 mg/kg, s.c) for two consecutive days. The effect of prophylactic treatment was analyzed by estimation of electrocardiographic changes, serum biomarker levels, antioxidant level in tissue and histopathological report. Green tea in both high and low dose and their combination groups with hydrochlorothiazide significantly reduced the serum biomarker levels, increased antioxidant levels in tissue, restored the electrocardiographic changes compared to isoproterenol only treated group. The combination group showed substantial amount of restoration of all the parameters compared to hydrochlorothiazide alone treated group. To conclude the combination of green tea extract with hydrochlorothiazide reduced the side effect and found to be effective against myocardial stress.