METHOD DEVELOPMENT AND VALIDATION FOR DETERMINATION OF ZIDOVUDINE BY UV-SPECTROPHOTOMETER

Abstract

This paper describes validated Spectrophotometry method for determination of zidovudine in pure powder and formulation. The solutions of standard and sample were prepared in methanol. Quantitative determination of the drugs was performed at 266 nm for zidovudine. Proposed method was evaluated for the different validation parameters. The specificity test showed that there was no interference from excipients commonly found in the commercial pharmaceutical formulations at analytical wavelength of zidovudine. Quantification was achieved over the concentration range 1-10 $\mu$ g.mL 1 with mean recovery of 98.26 ± 0.84. This method is simple, precise, and sensitive and applicable for the determination of zidovudine in pure powder and formulation. The method was compared to high performance liquid chromatography (HPLC) method, which was reported for the same drug. No significant difference was found between the methods for zidovudine quantitation.