HEPATOPROTECTIVE ACTIVITY OF YAKRIT PLIHANTAK CHURNA AGAINST CARBON TETRACHLORIDE INDUCED OXIDATIVE STRESS AND HEPATOTOXICITY IN WISTAR ALBINO RATS

Abstract

The objective of this study is to evaluate the hepatoprotective effect of the Yakrit plihantak churna against CCl4 induced hepatotoxicity in Wistar albino rats. The methods are hepatoprotective activity was assessed using CCl4 induced hepatic injury in rats by monitoring the biochemical parameters along with oxidative stress markers. Biochemical markers of hepatic injury such as aspartate transaminase (AST), alanine transaminase (ALT), alkaline phosphatase (ALP), lactate dehydrogenase (LDH). The liver function was assessed by monitoring the level of total bilirubin, total protein and levels of albumin in serum. The oxidative stress was evaluated by measuring the levels of Malondialdehyde and superoxide dismutase. The results are CCl4 and olive oil mixture of (1:1 dosage of 1 ml/kg b.w) induced oxidative stress was indicated by elevated levels of AST, ALT, ALP, LDH, total bilirubin and MDA. The depleted levels of total protein, albumin and superoxide dismutase were found in CCl4 induced animals. The Yakrit plihantak churna at two dose levels 200 mg/kg and 400 mg/kg significantly re-established the hepatic parameters towards normal level as compared to the elevated biochemical markers enzymes in the CCl4 treated animals. Reversal to normal tissue architecture was observed in histological evaluation. The results of YPC (400 mg/kg) were found comparable with that of the standard drug silymarin in all the parameters. The conclusion is the above findings suggest the therapeutic potential of these plants in alleviating hepatic oxidative stress and tissue damage. This shows its hepatoprotective activity. Further in-vivo studies are needed to explore the possible mechanism of action of isolated compounds derived from Yakrit plihantak churna in treating liver diseases.