ISOLATION AND IDENTIFICATION OF L-GLUTAMINASE AN ANTILEUKEMIC ENZYME PRODUCING MICO-ORGANISM FROM GODAVARI RIVER BANK SOILS IN ANDHRA PRADESH
Keywords:
L-Glutaminase, Isolation, Identification, 16s rDNA gene, Biochemical characterization, Godavari river bankAbstract
Considering at the potential application of the L-glutaminase in pharmaceutical, food industries and chemical industry, a L-glutaminase producing micro organism was isolated from the soil samples of Godavari river bank of Andhra Pradesh, India. Among several isolates, one isolate (RSP-GLU) exhibited the highest L-glutaminase activity. The isolate RSP-GLU was identified based on the morphological, physiological and biochemical properties. Further 16S rDNA sequence (GenBank Accession Number AM990996) based phylogenetic analysis conformed that the isolated strain RSP-GLU as Bacillus subtilis.
