SENSITIVE AND SELECTIVE ANALYTICAL METHOD FOR THE QUANTIFICATION OF GLIPIZIDE IN HUMAN PLASMA

Abstract

A sensitive and selective high performance liquid chromatographic method for determination of Glipizide in Human plasma has been developed. The plasma samples were acid buffered, and samples were extracted with benzene, and organic layer was evaporated to dryness. The residue was reconstituted in mobile phase 0.05M Potassium Dihydrogen Orthophosphate: Methanol [15: 85 %v/v, pH 7.0 ± 0.05, pH adjusted with 1% Triethylamine] and an aliquot of 20 $\mu$ L was chromatographed on C Intersil [4.6(id) x 250 mm] column. Quantification was achieved by monitoring the ultraviolet absorbance at 225nm. The retention time of Glipizide was found to be 3.21 min and the response was linear (10-2000 ng cm) and detection limit was 5ng cm in plasma. The developed method was validated in terms of accuracy, precision, linearity, limit of detection and limit of quantitation and can be used for the estimation of these drugs in pharmaceutical dosage forms.