ANALYSIS OF BIOACTIVE COMPOUNDS OF HEMIONITIS ARIFOLIA (BURM.) MOORE. AN ANTI-DIABETIC FERN USING GAS CHROMATOGRAPHY AND MASS SPECTROSCOPY

Abstract

Hemionitis arifolia (Burm.) Moore. is an important medicinal plant belongs to the family Hemionitidaceae. In Asian folklore Hemionitis arifolia is used to treat diabetes. The fern has been medically evaluated for its anti-diabetic properties and for intestinal worms. The present work is an important exploration and aims at analysing ethanolic extract of the plant using Gas Chromatography-Mass Spectrometry. The mass spectra of the compounds found in the extract was matched with the National Institute of Standards and Technologies (NIST) library. Gas chromatography mass spectrometry (GC-MS) analysis revealed the presence of nine compounds and the phyto constituents screened were 1. 4-(chloromethyl)-1-azabicydo-(2.2.2)octane (18.26%), 2. 4-Nitrobenzoic acid, 2,4,6-trichlorophenyl ester (18.70), 3. Decanoic acid, 2-hexyl-,methyl ester(19.91), 4. N-(4-(-

Chlorophenyl)isothiazol-5-yl)-2-methylpiperidin-2-imine (21.73), 5. Pregnane-3,20-dione, 17,21-{(methylborylene)bis(oxy)}-,5a- (23.76), 6. Retinoic

acid, 5,8-epoxy-5,8-dihydro (24.80), 7. Androst-5-en-3-one, 19-acetoxy-4, 4-dimethyl-, oxine (26.14), 8. Choestan-26-oic acid, 3,7,12-trihyroxy-, (3a,5a,7a, 12a) (28.07), 9. Benzamide, N-(4-chlorotetrahydro-3-thinyl)-3, 5- dimethoxy, s-dioxide (31.52). The nine compounds identified provide positive light upon anti-diabetic works and drug could be formulated in future for diabetic condition. Hence Hemionitis arifolia is an important medicinal material for further research leading to possible drug development.