ANTIOXIDANT AND ANTICANCER ACTIVITY OF LAGENARIA SICERARIA FRUIT EXTRACTS ON MCF-7 CANCER CELL LINES

Abstract

Object: To evaluate the anticancer and antioxidant activity of Lagenaria siceraria. Materials and methods: The different extracts of Lagenaria siceraria were studied for anticancer activity against MCF-7. The inhibitory properties of these extracts are compared with standard 5-Fluoro Uracil for MCF-7 cell line. The Percentage cancer cell inhibition profiles were found to be concentration dependent. The maximum concentration (µg/ml) used in the study was 80 µg/ml and the extracts were also studies for antioxidant activity by DPPH radical scavenging activity. Results: The ethanolic extract of Lagenaria siceraria shows the better results as compared to other extracts and in phenol and Flavonoid content estimation ethanolic extract showed the better results. The Phytochemical investigation showed the presence of phenol, flavonoid, glycosides, alkaloids Saponins. Conclusion: The existence of phenolic and flavonoid compounds in the extract may be accountable for the anticancer and antioxidant activity. Thus this activity can be contributed to the phytochemicals present in it. The ethanolic extract of Lagenaria siceraria can be concluded to possess highest amounts of phenolic, flavonoid and DPPH free radical scavenging activities from the present studies.