POTENTIAL ACTIVITY OF MADHUCA LONGIFOLIA AGAINST MSU-INDUCED ARTHRITIS IN FEMALE WISTER ALBINO RATS

Abstract

Gout is a well-known form of arthritis which is caused due to deposition of monosodium urate crystals on joints throughout the body. Madhuca longifolia is evergreen tree that is known to have anti-arthritic activity. The aim of this study is to evaluate the Anti-arthritic activity on female Wistar albino rats by using aqueous extract of Madhuca longifolia leaf and its different patterns of interaction between receptors responsible for gout. Animal experiment was carried out with 30 female rats. MSU was induced on Group II, Group III and Group IV. In which Group III received aqueous leaf extract of Madhuca longifolia. After the study duration the rats were tested for the level of renal enzyme markers, renal antioxidant and renal histopathology. In silico docking analysis was carried out with the active components of Madhuca longifolia between receptors responsible for gout. The study was done by using Patchdock server for docking and the docked files were visualized and analyzed by using Pymol software. MSU has caused abnormalities in renal enzyme markers, antioxidant assays and histopathology. The rats treated with Madhuca longifolia were able normalize the toxicity caused by MSU which is similar to Indomethacin. The rats treated with Madhuca longifolia were able normalize the toxicity caused by MSU which is similar to indomethacin. The in-silico docking analysis has shown best binding affinity with Myricetin-3-O-arabinose with 9 bonds.