STUDIES ON DIFFERENTIAL PULSE POLAROGRAPHIC METHOD DEVELOPMENT AND VALIDATION OF RIBOFLAVIN IN VITAMIN TABLET: COMPARISON WITH UV-VIS SPECTROSCOPY AND PHOTO FLUORIMETRY

Abstract

A deficiency of riboflavin occurs only when the diet is very poor and lacks many nutrients. A lack of riboflavin causes sores in the mouth and inflammation of the tongue. Several methodologies have been developed for the determination of riboflavin, but those methods have few disadvantages like long analysis time, consume a lot of reagents and expensiveness. Hence, to overcome these problems, the differential pulse Polarographic technique was proposed in this study. The DPP method was used for the quantitative analysis of riboflavin. The experimental conditions were optimised to obtain the best characterised peak in terms of peak height with analytical validation of the method. This method is compared with UV-VIS spectroscopy and photo fluorimetry. It was found that the proposed method is highly sensitive, precise and fast.