EVALUATION OF NEWER POLY HERBAL FORMULATIONS FOR WOUND HEALING AND ANTI-INFLAMMATORY ACTIVITY

Abstract

According to Herbalism, independent medicinal herbs are not sufficient to obtain the required pharmacological effect, but when we use these herbal
compounds in combination at appropriate proportions will help to obtain maximum pharmacological effects with minimal toxic effects. In this present
study, efforts were made to develop an intervention with a poly herbal formulation which was prepared from ethanolic leaf extracts of Chromolaena
odorata, Tridax procumbens, Euphorbia hirta Linn. The impact of poly herbalism is assessed for its wound healing property by using excision wound
model and anti-inflammatory property by using formalin induced paw oedema model. For wound healing assessment two polyherbal formulations F1
and F2 are prepared with 2% and 4% W/W. These formulations were examined for stability studies. F1(*p<0.005) and F2 (**p<0.001) have shown
significant wound closure and faster epithelialisation when compared to standard Povidone-Iodine (5%) ointment (**p<0.001). For assessing antiinflammatory activity, the poly herbal formulations are prepared as 2% (F1) and 4% (F2) solutions. The doses from each formulation are given as 100
mg/kg, 200 mg/kg and 300 mg/kg to different groups of animals. Both the poly herbal formulations have shown significant results F1(*p<0.05,
**p<0.005) and F2 (***p<0.0005, ****p<0.0001) respectively when compared to standard Indomethacin 4 mg/kg dose (****p<0.0001). This study
has revealed that poly herbal formulations have shown better anti-inflammatory and wound healing properties due to the synergism of the
phytochemicals present in these plant extracts which may lead to future developments of potential poly herbal formulations in the treatment of wounds
and inflammatory mediated diseases.