PROTECTIVE ACTIVITY OF ALPHA-PINENE AND TRANS-ANETHOLE AGAINST 3-NITRO PROPIONIC ACID INDUCED NEUROTOXICITY IN ANIMAL MODEL OF HUNTINGTON’S DISEASE

Abstract

Huntington’s disease (HD) is a highly destructive autosomal dominant neurodegenerative disorder. 3-Nitropropionic acid (3-NP), model is a well- known model to induce Huntington’s disease (HD) in rodents. It is a neurotoxin which causes severe neurotoxicity in the form of oxidative stress. Alpha-pinene (AP) and Trans-anethole (TA) are the natural compounds having mainly antioxidant and neuroprotective effects. In the present study, the neuroprotective effect of Alpha-pinene and on 3-NP induced oxidative stress in rat striatum was determined by behavioural and biochemical parameters. Rats were induced with 3-NP (10 mg/kg) intraperitoneally for 14 days and rats induced with 3-NP were treated with Alpha-pinene and Trans-anethole

- (50 mg/kg, each, per. oral) and the combination group (AP + TA) - (25 mg/kg, each, p. o) for 14 days. Alpha-pinene and Trans-anethole have given better results in the case of body weight reduction and behavioural analysis which includes (elevated plus maze (Anxiety), forced swim test, rota rod activity (grip strength). Biochemical estimation includes (lipid peroxidation (MDA), SOD, GSH, Catalase levels), It decreases the increased level of MDA and increased the decrease level of SOD, GSH and Catalase levels), while combination effect of Alpha-pinene and Trans-anethole were found to be more effective. The present study shows that the antioxidant activity of alpha-pinene and trans-anethole may be responsible for its neuroprotective activity against 3-nitropropionic acid induced neurotoxicity in rats.