PHYLOGENETIC ANALYSIS, OPTIMIZATION OF NUTRITIVE MEDIA COMPOSITION AND IMMOBILIZATION OF XYLANASE ENZYME PRODUCED BY BACILLUS LICHENIFORMIS STRAIN SC

Abstract

Enzyme productions by microorganisms are increasing with rising cost and finite supply of fossil energy. Microorganisms required for the conversion of lignocellulosic materials into fermentable sugars. A newly Bacillus was isolated from decaying wood and identified for high xylanase activity. It was identified as Bacillus licheniformis strain (KJ842626) by 16S rRNA gene sequencing and by different biochemical tests. The phylogenetic relations to other xylanolytic bacteria were also analyzed. The immobilization of enzyme had been done by gel entrapment process with final concentration of 4 % agar and 1 % agarose solution. The immobilized enzyme might be advantageous for industrial applications therefore the immobilization of cells for higher xylanase production. From the above investigations it was concluded that xylanase produced by Bacillus has industrially application in different industry and the cell entrapment composed of agar and agarose exhibited higher activity and better operational stability.