PHYTOCHEMICAL SCREENING, CYTOTOXIC AND HYPOGLYCEMIC ACTIVITY OF METHANOLIC EXTRACT OF CITRUS SINENSIS PEEL

Abstract

Orange has been traditionally used as an adjuvant in different diseases including diabetes, tuberculosis, asthma and hypertension. An examination of the impacts of peel extract from Citrus sinensis in hyperglycemic Albino mice uncovered the glucose lowering activity of Citrus sinensis. In this study, the methanolic extract of the fruit peel was assessed in two dosage regimen- 200mg/kg and 400mg/kg, regarding the regulation of glucose incited diabetes mellitus. Both doses were being found to reduce the serum glucose level significantly (P value<0.0001) by 32% and 42%, respectively after 120 minutes of administration; revealing the anti hyperglycemic activity of the methanolic extract of Citrus sinensis peel. In the Brine Shrimp Lethality Toxicology study, the LC50 value of the extract was found 17.885µg/ml which indicated minimum lethal effect compared to that of standard (vincristine sulfate) which was 1.283µg/ml and the phytochemical screening of the methanolic extract of the peel showed presence of different bioactive compounds like flavonoids, saponins, tannins, alkaloids which are health promoting secondary metabolites.