PREPARATION, CHARACTERIZATION AND OPTIMIZATION OF PRONIOSOMAL GEL OF ANTI-INFLAMMATORY AGENT

Abstract

Proniosomal gel of anti-inflammatory agent (Diclofenac Sodium) was developed using two different non – ionic surfactants (Span 60 and Span 40) with an objective to develop stable, robust, controlled release gel of diclofenac sodium as it has short half-life and narrow therapeutic index. Various proniosomal gels were developed using different concentration of nonionic surfactants and one formulation was selected as optimized formulation based on the size, shape and % entrapment efficiency. FS603 was selected as an optimized formulation and was characterized further for zeta potential and stability and was found as stable at both refrigerated and room temperatures. No edema and erythema were observed in rats treated with formulation FS603 for four days and skin irritation index found as zero. In vivo anti-inflammatory activity with optimized formulation was carried out in rats against marketed preparation using carrageenan induced rat paw method and shown greater % inhibition of inflammation and extended period when compared with marketed preparation.