TEST OF ANTIOXIDANT ACTIVITY LEAVES OF SCAEVOLA TACCADA (GAERTN.) ROXB. USING DPPH (1, 1-DIPHENYL-2-PICRYLHYDRAZYL)

Abstract

Free radicals can caused damage cell and tissue which lead to various disease then needed antioxidant as radicals scavenging. The objective of this study was to determine antioxidants activity leaves of Scaevola taccada (Gaertn.) Roxb based to DPPH (1,1-diphenyl-2-picrilhydrazil) free radicals scavenging. The study was initiated with sample extraction by maceration used ethanol then partitioned with ethyl acetate. The obtained fraction tested to antioxidant activity DPPH radicals. Testing done in five series concentration is 50 ppm, 100 ppm, 200 ppm, 300 ppm and 400 ppm, which compared to antioxidant activity vitamin C and BHT (butylatedhydroxytoluene). Activity against free radicals is measured with at a spectrophotometer UV-VIS wavelength 517 nm and calculated value ES50. The result showed the value ES50 of a leaves ethyl acetate fraction of 1473 ppm while ES50 vitamin C and BHT of 9.054 ppm and

29.067 ppm, respectively. Therefore, obtained results showed that ethyl acetate fraction Scaevola taccada (Gaertn.) Roxb leaves has weak activity compared vitamin C and BHT as positive control with ES50 values respectively 1473.064 ppm, 9.054 ppm and 29.067 ppm