EVALUATION OF SILVER NANOPARTICLES AS A COMPARATIVE STUDY AND DETERMINATION OF ITS EFFECT ON VIGNA RADIATA

Abstract

The silver nanoparticles (AgNP’s) were synthesized using green synthesis and chemical synthesis method. The nanoparticles synthesized using green synthesis (boiled extract and normal extract) were compared for the difference in various properties after characterization using X-ray diffraction (XRD), Fourier Transform Infra-Red spectroscopy (FT-IR) and Atomic Force Microscopy (AFM). There is not much difference between the AgNP’s synthesized using both the methods. The chemically synthesized AgNP’s were also characterized. FTIR analysis revealed that there is a strong peak of absorbance at 1379 cm-1 on comparison with the control. XRD analysis is used to find the nature and size of the silver nanoparticle. The silver nanoparticles are found to be more stable in distilled water than the deionized water and tap water. The antimicrobial activity is high for the boiled extract sample when compared with other samples. The effect of AgNP’s on Vigna radiata is studied by exposing the seeds in time intervals and allowing them to germinate. The comparison was done by estimation of chlorophyll and protein. Based on the studies it could be said that if the exposure to silver nanoparticles increases the content of the plant will be affected.