CHROMATOGRAPHIC DETERMINATION OF PHENOLIC PROFILE FROM PUNICA GRANATUM FRUIT PEELS

Abstract

Polyphenols are the important active compounds present in the fruits peels of Punica granatum. The objectives of this study were to isolate and identify the phenolic compounds by UV- spectrophotometry, thin layer chromatography (TLC), high-performance liquid chromatography (HPLC) and liquid chromatography - mass spectrometry (LC- MS) methods for qualitative and quantitative analyses of phenolic compounds in fruit peels of

1. granatum. For separation of bioactive compounds, the crude ethanolic extracts of P. granatum were fractionated by using column chromatography. These fractions were coded as PG I, PG II, PG III, PG IV and PG V for P. granatum. These fractions were analyzed by thin layer chromatography (TLC) and showed that PG II fraction had an interested compound. The highest total phenolic and flavonoid content were observed in the PG II fraction of P. granatum (617 ± 0.017 mg/g and 546.33 ± 0.032 mg/g respectively) in comparison to other fractions. The phenolic compounds gallic acid and caffeic have been identified in the PG II fraction of P. granatum by using different chromatographic techniques i.e. TLC, HPLC, LC- MS analysis. The overall results of the present studies were indicated that PG II fraction of P. granatum was a good source of phenolic compounds. The present analytical study proved a potential application to identify and quantify the phenolic compounds in the plant fractions. Hence, it could be pursued further for obtaining phytomedicine.