EVALUATION OF CARDIOPROTECTIVE ACTIVITY OF FULVIC ACID AGAINST ISOPROTERENOL INDUCED OXIDATIVE DAMAGE IN RAT MYOCARDIUM

Abstract

The aim of study was to evaluate cardio protective activity of fulvic acid in Isoprenaline induced cardiac toxicity. In current investigation, female/male wistar rats were divided into five groups, normal, control, Fulvic acid 100mg/kg, Fulvic acid 200mg/kg, and Fulvic acid 300 mg/kg. Isoprenaline 85 mg/kg was administered on 29^th and 30^th day during study period to all except normal group. Fulvic acid was administered to respective groups once daily for total 30 days, on the last day of study, the animals were anesthetized to record ECG and BP (by cannulating carotid artery), blood was collected from carotid artery and SGOT, LDH And CK-MB were estimated, Animals were sacrificed to isolate heart and preparation of tissue homogenate. The antioxidant status is analyzed by measuring MDA content, SOD CAT, GSH activity. The tissue sample is also preserved for histological studies. Isoprenaline causes cardiac damage which was manifested by alteration in serum cardiac markers, antioxidant markers, ECG and hemodynamic and histological changes. These alterations were restored due to treatment with fulvic acid 300mg/kg. With data obtained in study it have been concluded that fulvic acid treatment for 4 weeks protect the heart of rat from cardiotoxicity as a result of isoprenaline administration.