ANTIOXIDANT ACTIVITY OF RAPHANUS SATIVUS L. OF JHANSI DISTRICT, UTTAR PRADESH, INDIA

Abstract

Raphanus sativus L. commonly known as Muli, belongs to the family Brassicaceae. Production of Reactive Oxygen Species (ROS) causes various diseases and cellular anomalies in human beings. Antioxidants inhibit generation of reactive species, or scavenge them, or raise the levels of endogenous antioxidant defenses. Keeping in view of the above beneficial effects of Muli, we shought to analyse the phytochemicals present in the quath, aqueous and methanolic extract. We collected Muli from the local market of Jhansi and washed with tap water and finally with distilled water. After drying the radish leaves at room temperature, it was crushed and was used for aqueous and methanolic and quath extractions. Phytochemical analysis of the leaves and roots of R. sativus L. var niger had most of the important phytoconstituents like Alkaloids, Tannins, Cardiac glycosides, Terpenoids, Flavonoids, Steroids are present in the methanolic extract of radish leaves while in quath and aqueous extract Alkaloids, Flavonoids, Glycosides, Tannin & Phenolic compounds, Saponins, Amino acids and Terpenoids are present in most of the test. As compared to leaves, radish root shows less phytochemical constituents in all the extract. We observe the antioxidant activities in both radish leaves and roots. We conclude that there was no effect of concentration on phytochemical constituents. Radish leaves have more phytochemical constituents while radish roots have less. Further, radish leaves show more antioxidant activities as compared to roots. However, methanolic extraction is superior to aqueous extraction for the study of phytochemical constituents.