EVALUATION OF IN VITRO ANTI-COAGULANT AND IN VITRO ANTI DIABETIC ACTIVITY OF ALSTONIA SCHOLARIS LEAVES

Abstract

This study was conducted to evaluate in vitro anti-coagulant and in vitro anti diabetic activity of ethanolic extract of Alstonia scholaris leaves. For in vitro anti-coagulant activity we using blood samples, blood was centrifuged and plasma was used, in this method 0.2 ml of plasma is incubated with

0.1 ml of 200 and 400ug/ml plant extract and 0.3ml of 25mM cacl2 solution and time for coagulation is recorded and for in vitro antidiabetic activity we estimate the alpha amylase inhibition and alpha glucosidase inhibition methods and percentage of inhibition was calculated. Anti-coagulant activities of ethanolic extract of Alstonia scholaris were carried out, 400ug/ml solution delayed the coagulation time for 26.56 min. There was a dose- dependent increase in percentage inhibitory activity against alpha- amylase enzyme. At a concentration of 0.2 ml of plant extract showed a percentage inhibition 27.8% and for 1.0 ml plant extract showed inhibition of 96.3% .The Alstonia scholaris ethanol extract revealed a significant inhibitory action of alpha-glucosidase enzyme. The percentage inhibition at 0.2-1.0 ml concentrations of Alstonia scholaris extract showed a dose dependent increase in percentage inhibition. The percentage inhibition varied from 89.4% - 31.7% for highest concentration to the lowest concentration. In this present study we evaluated in vitro anti-coagulant, in vitro alpha amylase and alpha glucosidase activity of crude methanol extract of Alstonia scholaris leaves. The plant showed significant inhibition activity.