DEVELOPMENT AND VALIDATION OF RP-UPLC METHOD FOR SIMULTANEOUS ESTIMATION OF CARVEDILOL AND HYDROCHLOROTHIAZIDE IN PHARMACEUTICAL DOSAGE FORM

Abstract

The aim of the present study was to develop and validate a simple, novel, rapid isocratic ultra-performance liquid chromatography for simultaneous estimation of Carvedilol and Hydrochlorothiazide in combined dosage form using Hypersil gold Column - C18 (50 X 2.1mm) 1.9 micron with Mobile phase composition of Water, methanol, and Acetonitrile in the ratio of 30:20:50 (pH 5.5). The flow rate of 0.3 ml/ min and UV detection at 285 nm was maintained during entire study. The retention time of hydrochlorothiazide and Carvedilol were found to be 2.06 and 2.39 mins respectively. All validation parameters were evaluated as per ICH guideline, which remained well within the acceptable limit. This proposed method can be used for estimation of Carvedilol and Hydrochlorothiazide in bulk and pharmaceutical dosage forms