ISOLATION, CHARACTERIZATION, ANTIMICROBIAL POTENTIAL OF ENDOPHYTIC FUNGUS FROM ANETHUM GRAVEOLENS AND ITS INSILICO VALIDATION

Abstract

The research was mainly focused to identify bioactive endophytic fungus which serves as an alternative source of alkaloids produced by their hosts. Fungal diversity interprets significant conservation strategies and its medicinal importance. Although medicinal herbs serve against many ailments, fungal endophytes in today’s scenario play a vital role in acting against certain infections but their mode of distribution and pattern of growth are still unexplored. In this research an attempt was made to isolate endophytic fungus from leaves of Anethum graveolens and to determine its antimicrobial potential along with molecular characterization studies thereby developing insilico docking interactions for the compounds that was quantitatively analyzed by GC-MS. The results revealed that antimicrobial resistance was significant against methicilin resistant Staphylococcus aureus. The molecular identification of the strain confirmed that it belongs to Colletotrichum sp which comes under Glomerellaceae family. The bioactive compound in the metabolite was found to be 2,2-Bis[4-[[4-chloro-6-(3-ethynylphenoxy)-1,3,5-triazin-2-yl]oxy]phenyl]propane which exhibited maximum peak. The compound showed best docked pose with least binding energy -117.142 kcal/mol and binding was found to be predominant in active site of MRSA receptor. Thus the outcome revealed significant antimicrobial activity against MRSA which provides a suitable drug target using iGEMDOCK and the metabolite isolated has a potential source on its own.