IN VITRO ANTIOXIDANT AND ANTIMICROBIAL POTENTIAL EFFECT OF MURRAYA KOENIGII ROOT EXTRACT AGAINST MULTI DRUG RESISTANT HUMAN PATHOGENS

Abstract

To investigate the phytochemical screening, antibacterial, antifungal and antioxidant activity of the consecutive extract of Murraya Koenigii Roots (called as MKR). This research work comprises four different crude extract and it is mainly focused on the extract of MKRS2.The phytochemical screening for all the crude extract was carried out by the standard method. The antimicrobial activities of MKRS2 extract were evaluated by disk diffusion method. The antioxidant activity of MKRS2 was the determination of DPPH, FRAP and ABTS at different doses and MKRS2 was characterization by TLC, FT-IR and LC-MS. The antimicrobial activity of MKRS2 showed promising effective activities against all the bacterial and fungal pathogens with a maximum zone of inhibition against E.coli 20±1.5 mm and Fusarium rhizopus 20±1.8 mm. The MIC values of antibacterial were shown the maximum range at 150µg / mL and 300µg / mL antifungal activity. The antioxidant activity of MKRS2 exhibits the IC50 values ranging from 24.6 ± 0.8, 27.5 ± 2.2 and 37.7 ± 3.0.The phytochemical constituent’s alkaloids, tannins, saponin, carbohydrates, glycosides and oils were present in all the crude extracts.The TLC studies showed the presence of four different UV-active fractions. The FT-IR spectrum indicates the presence of possible functional groups O-H, C=C and N-H starching of aromatic conjugates. The LC-MS of the MKRS2 shows m/z 263 and 332 two different peaks with responsible intensity. These finding shows evidence of MKRS2, an excellent antimicrobial and antioxidant activities and have great potential as a source for natural health care products.