STUDIES ON PROXIMAL COMPOSITION AND DNA BARCODING OF MARINE SHRIMPS FROM THONDI, TAMILNADU, INDIA

Abstract

The shrimp is one of the most popular species as it is almost a part of every nation’s traditional meal, rich in protein and minerals. Because of their
many nutritional benefits, prawns are considered to be the healthiest foods in the world. They also have great economic value as they earn valuable
foreign exchange. Four different types of marine shrimps were identified morphologically and molecularly discriminated by mitochondrial COI
(cytochrome C oxidase subunit) gene. DNA barcoding is useful tool for identification and potential discovery of new species. The investigation
principally entails with the proximate analysis such as moisture, ash, protein, carbohydrate, lipid and fatty acid content of four different types of
marine shrimps (Type I - Grooved tiger shrimp, Type II - Mangrove or snapping shrimp, Type III - Glass shrimp and Type IV - Squilla mantis
shrimp). The results showed that the high amount of moisture (72%), Protein (190.19 µg/g) and Lipid (167 µg/g) content were found to be in Type III
marine shrimps compared to other three samples. Of these four sample shrimps, Ash (6.71 %), Carbohydrate (184 µg/g) and fatty acid (154 µg/g)
content were found to be higher in Type IV sample. The study reconfirms the usefulness of DNA barcoding for the identification of marine shrimps.
BOLD system analysis accurately identifies the species name and their similarities were checked with BLAST-NCBI. Each and every species showed
80% identity.