STEAM DISTILLATE OF MURRAYA KOENIGII AS A TYROSINASE ACTIVATOR

Abstract

Tyrosinase (E. C. 1. 14. 18. 1) is ubiquitous enzyme involved in pigmentation that catalyses hydroxylation of monophenol's and oxidation of phenols in presence of molecular oxygen. Deterioration in tyrosinase activity is responsible for vitiligo. In current research we have isolated tyrosinase from germinating jackfruit (Artocarpus heterophyllus) seeds and partially purified it using ammonium sulphate (30%) precipitation, and this partially purified enzyme had been used as an enzyme source. Steam distillates of six plants extracts namely Murraya koenigii, Chrysopogon zizanioides and Phyllanthus emblica , Pandanus odorifer, Santalum album, Rosa rubiginosa were studied for tyrosinase activation activity. Out of that 100µg of Vetiveria zizanoides and Phyllanthus emblica increases activity by 2.5 times and Murraya koenigii (MK) by 3 times. So extract of MK (curry leaf) were studied in detail. Enzyme kinetic study showed that Murraya koenigii is mixed to non-competitive kind of activator. We had further done TLC and active purified constituent used for FTIR study. FTIR structure shows that it contains aromatic amines, activator concentration beneficial to increase tyrosinase activity in different industry. Over all this activator has lots of important in industry and pharmaceutical science.