EVALUATION OF ANTIBACTERIAL EFFICACY AND WOUND HEALING ACTIVITY OF NATURAL FIBRE BASED WOUND DRESSING COATED WITH NATURAL EXTRACTS OF CALOTROPIS GIGANTEAN, EUCALYPTUS GLOBULES AND BUDS OF SYZYGIUM AROMATICUM ENHANCED WITH EPIDERMAL GROWTH FACTOR

Abstract

In this work, the leave extracts of Calotropis gigantean, Eucalyptus globules and buds of Syzygium aromaticum were prepared and coated on natural
fibres based bamboo gauze fabric for wound dressing applications. The fabrics coated with different compositions of the natural extract were
enhanced with rhEGF (REGEN-D™ 60). The antibacterial activity of the developed samples was studied after the identification of its mechanical
properties and Scanning Electron Microscopy. The wound healing efficacy of the coated fabric samples were studied through In vivo method using
Wister Albino rats. The type of wound selected for the study was second degree burn wound. The dorsal surface of the rat’s skin was removed and
second degree burn wound was created. Then the developed fabric samples were dressed over the wound surface and the wound healing was studied
by wound closure rate in equal interval of days. A specified composition of natural extracts coated on bamboo gauze fabric enhanced with rhEGF
(REGEN-D™ 60) exhibits good antibacterial property was confirmed from the formation of zone of inhibition in antibacterial test against Gramnegative (Escherichia coli) and a Gram-positive bacterium (Staphylococcus aureus) and appropriate wound closure rate on second degree burn wound
through In vivo evaluation. Hence therefore it was concluded that coated bamboo gauze fabrics can be suitable for faster burn wound healing process.