ASSESSMENT OF IN VITRO ANTI-OXIDANT AND ANTI-MICROBIAL EFFICIENCIES OF ENDANGERED MEDICINAL PLANT FICUS DALHOUSIAE MIQ.

Abstract

Ficus dalhousiae Miq, is sparely populated endangered plant of Nilagiri hills and eastern ghats of India. It was reported to have prominent medicinal activities in the traditional medicinal system. The successive soxhlet extraction method was carried out using Hexane, Acetone, Methanol and Aqueous solvents. The quantitative estimation of total phenol content was 94µg GAE/µg by folin ciocalteu method. The antimicrobial activity was moderate and dose dependent. Polarity of the solvent was directly proportional to the zone of inhibition. Antioxidant activity of the four extracts was calculated and their IC50 values were compared with the standard ascorbic acid. Methanol extract was found to have 91.36 µg/ml inhibitory concentrations. The IC50 values were significantly increased for the four extracts and the percentage of inhibition was directly proportional to the concentration of the extract. Methanol extract has the effective antimicrobial activity and antioxidant activity.