OPTIMIZATION OF ALPHA AMYLASE EXTRACTED FROM MARINE ACTINOMYCETES-STREPTOMYCES GANCIDICUS_ASD-KT852565
Keywords:
Andaman & Nicobar island, Marine soil sample, Actinomycetes,, Alpha amylase,Abstract
The crude extract obtained from Streptomyces gancidicus_ASD-KT852565 isolated from marine actinomycetes soil sample which was collected from Andaman & Nicobar Island was subjected to optimization with 10 different parameters like temperature, Nitrogen source, Carbon source etc. for better yield of alpha amylase. The results obtained showed that the enzyme production by S.guancidicus_ASD-KT852565 was optimum at temperature 40°C, pH 7.0, with carbon source as sucrose, Nitrogen source as Beef extract, Amino acid as L-asparagine and also at NaCl concentration of 1.5 %, volume of inoculum as 50ml in 250 ml flask, incubation time maintained for 96 hours enhanced with MgSo4 as metal ion and finally starch as substrate at the concentration of 50g/L showed good yield applicable for industrial use.
