ENZYMATIC AND NONENZYMATIC ANTIOXIDANT ACTIVITIES OF Cleome viscosa AND Cleome gynandra WHOLE PLANT METHANOLIC EXTRACTS IN STREPTOZOTOCIN-INDUCED DIABETIC RATS

Abstract

This study was designed to scrutinize the enzymatic and nonenzymatic antioxidant activities of the methanolic extract of whole plant of Cleome viscosa and Cleome gynandra (MeCV and MeCG) in streptozotocin (STZ) induced diabetic rats. Oral administration of MeCV, MeCG and Me.CV+CG combination at a dosage of 400 mg/ kg b.w for 28 days on diabetic rats. The enzymatic and non-enzymatic antioxidant assays were measured by using standard procedures in liver tissue of diabetic and normal rats. There is a significant (p < 0.05 and p < 0.01) improvement in the activities of superoxide dismutase (SOD), catalase (CAT) and glutathione - s-transferase (GST) in liver tissue of STZ induced diabetic treated rats when compared with the untreated diabetic rats and the contents of ascorbic acid (AA) and glutathione (GSH) levels were also significantly increased in the treated diabetic rats when compared with untreated diabetic rats. These results clearly indicated that the whole plants of MeCV have good hepato protective activity than MeCG whole plant in STZ induced diabetic rats.